Ultrasensitive Detection and Phenotyping of CD4+ T Cells with Optimized HLA Class II Tetramer Staining
نویسندگان
چکیده
منابع مشابه
Ultrasensitive detection and phenotyping of CD4+ T cells with optimized HLA class II tetramer staining.
HLA class I tetramers have revolutionized the study of Ag-specific CD8+ T cell responses. Technical problems and the rarity of Ag-specific CD4+ Th cells have not allowed the potential of HLA class II tetramers to be fully realized. Here, we optimize HLA class II tetramer staining methods through the use of a comprehensive panel of HIV-, influenza-, CMV-, and tetanus toxoid-specific tetramers. W...
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ژورنال
عنوان ژورنال: The Journal of Immunology
سال: 2005
ISSN: 0022-1767,1550-6606
DOI: 10.4049/jimmunol.175.10.6334